产品货号：
GS2222
中文名称：
MnlI内切酶
英文名称：
MnlI
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
相关搜索：MnlI内切酶，MnlI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CCTC(N)7↑ |
| 来源 | E.coli strain,that carries the cloned gene Mnl I from Moraxella nonliquefaciens |
| Assayed on | Lambda DNA |
| Optimal buffer | G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+?BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme 50%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping CG methylation:?CCTmCG. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
相关搜索：MnlI内切酶，MnlI限制性内切酶